DNA

Part:BBa_K2100034:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pERE5:TALER14


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 303
    Illegal EcoRI site found at 477
    Illegal XbaI site found at 454
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 303
    Illegal EcoRI site found at 477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 303
    Illegal EcoRI site found at 477
    Illegal BamHI site found at 600
    Illegal XhoI site found at 543
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 303
    Illegal EcoRI site found at 477
    Illegal XbaI site found at 454
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal EcoRI site found at 303
    Illegal EcoRI site found at 477
    Illegal XbaI site found at 454
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 615


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter and a gene from the mammalian genome.

References