![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100034:Design
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-17)
pEXPR pERE5:TALER14
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 271
Illegal EcoRI site found at 281
Illegal EcoRI site found at 303
Illegal EcoRI site found at 477
Illegal XbaI site found at 454 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 271
Illegal EcoRI site found at 281
Illegal EcoRI site found at 303
Illegal EcoRI site found at 477 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 271
Illegal EcoRI site found at 281
Illegal EcoRI site found at 303
Illegal EcoRI site found at 477
Illegal BamHI site found at 600
Illegal XhoI site found at 543 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 271
Illegal EcoRI site found at 281
Illegal EcoRI site found at 303
Illegal EcoRI site found at 477
Illegal XbaI site found at 454 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 271
Illegal EcoRI site found at 281
Illegal EcoRI site found at 303
Illegal EcoRI site found at 477
Illegal XbaI site found at 454 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 615
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
This is a synthetic promoter and a gene from the mammalian genome.